Volume 19, No. 5, 2022
Evaluation Of The In Vitro Antagonistic Potential Of Rice Plant-Associated Endophytic Bacteria Against Burkholderia Glumae
Alexander Pérez Cordero , Donicer E Montes-Vergara and Javier E Sierra
Abstract
Burkholderia glumae is the etiological agent of the disease called bacterial panicle blast of rice, which causes great economic losses in the agricultural sector. The objective of this research was to evaluate the in vitro antagonistic activity of endophytic bacteria isolated from rice varieties against Burkholderia glumae. A completely randomized design was applied for the in vitro antagonistic activity of endophytic bacteria against B. glumae and to establish differences between the population density present in rice varieties and the type of colonized tissue. Likewise, the Duncan multiple range test was used to establish significant statistical differences (p-value < 0.05) between colonized tissues and percentage of inhibition of endophytic bacteria. A total of 148 morphotypes of endophytic bacteria were isolated from the varieties F2000, FMocarí and F473. The F2000 variety showed a higher population density (p-value < 0.05). The root was the tissue with the highest population density of endophytic bacteria (p-value < 0.05) when compared to the rest of the tissues. The morphotypes H2M1LIM and P4M2LIM were molecularly identified by 16S rRNA gene as Burkholderia cepacia, R1M2LIM and R3M3LIM as Bacillus subtilis and T5M7 as B. cereus which showed in vitro inhibition against B. glumae. These species are characterized by the production of volatile and non-volatile secondary metabolites that have the ability to control growth in vitro against pathogens. The application of endophytic bacteria with antagonistic potential may become in the future a great alternative to replace the application of agrochemicals and chemical fertilizers.
Pages: 196-208
Keywords: agriculture, antagonism, Oryza sativa, production, yields.